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msu crystals  (InvivoGen)
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InvivoGen msu crystals
The effects of precursor polydatin (PD) and resveratol (RES) on calcium <t>pyrophosphate</t> <t>(CPP)</t> and monosodium urate <t>(MSU)</t> crystal-stimulated IL-1β production. Panel A, B: the cells were primed with PMA 100 ng/mL for 3 h and left overnight in fresh medium. The cells were then treated with CPP 0.025 mg/mL ( A ) or MSU 0.05 mg/mL ( B ) for 24 h in the presence of the two polyphenols. White bars show IL-1β extracellular concentrations, grey bars show IL-1β intracellular levels. In the left side of A and B figures are the control levels of IL-1β for each condition. Panel C: White bars show extracellular IL-1β level after crystal stimulation while grey and black bars show cytokine levels after 2 h cell pretreatment with PD (grey) and RES (black) followed by crystal stimulation. * p < 0.05 vs. CPP or MSU; ° p < 0.05 vs. basal control.
Msu Crystals, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effects of precursor polydatin (PD) and resveratol (RES) on calcium pyrophosphate (CPP) and monosodium urate (MSU) crystal-stimulated IL-1β production. Panel A, B: the cells were primed with PMA 100 ng/mL for 3 h and left overnight in fresh medium. The cells were then treated with CPP 0.025 mg/mL ( A ) or MSU 0.05 mg/mL ( B ) for 24 h in the presence of the two polyphenols. White bars show IL-1β extracellular concentrations, grey bars show IL-1β intracellular levels. In the left side of A and B figures are the control levels of IL-1β for each condition. Panel C: White bars show extracellular IL-1β level after crystal stimulation while grey and black bars show cytokine levels after 2 h cell pretreatment with PD (grey) and RES (black) followed by crystal stimulation. * p < 0.05 vs. CPP or MSU; ° p < 0.05 vs. basal control.

Journal: Foods

Article Title: Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells

doi: 10.3390/foods8110560

Figure Lengend Snippet: The effects of precursor polydatin (PD) and resveratol (RES) on calcium pyrophosphate (CPP) and monosodium urate (MSU) crystal-stimulated IL-1β production. Panel A, B: the cells were primed with PMA 100 ng/mL for 3 h and left overnight in fresh medium. The cells were then treated with CPP 0.025 mg/mL ( A ) or MSU 0.05 mg/mL ( B ) for 24 h in the presence of the two polyphenols. White bars show IL-1β extracellular concentrations, grey bars show IL-1β intracellular levels. In the left side of A and B figures are the control levels of IL-1β for each condition. Panel C: White bars show extracellular IL-1β level after crystal stimulation while grey and black bars show cytokine levels after 2 h cell pretreatment with PD (grey) and RES (black) followed by crystal stimulation. * p < 0.05 vs. CPP or MSU; ° p < 0.05 vs. basal control.

Article Snippet: CPP and MSU crystals were from InVivo Gen, Toulouse, France; phorbol myristate acetate (PMA), RPMI 1640, fetal calf serum, polymyxin B and phosphate buffer saline (PBS) were from Sigma-Aldrich, Milan, Italy.

Techniques: Control

The effect of PD and RES on IL-1β, NLRP3 and ASC gene expression. Expression of IL-1β ( A , B ), NLRP3 ( C , D ) and ASC ( E , F ) mRNA in THP-1 cells stimulated with crystals and treated (white bars) or pretreated (gray bars) with PD and RES. * p < 0.05 vs. CPP or MSU.

Journal: Foods

Article Title: Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells

doi: 10.3390/foods8110560

Figure Lengend Snippet: The effect of PD and RES on IL-1β, NLRP3 and ASC gene expression. Expression of IL-1β ( A , B ), NLRP3 ( C , D ) and ASC ( E , F ) mRNA in THP-1 cells stimulated with crystals and treated (white bars) or pretreated (gray bars) with PD and RES. * p < 0.05 vs. CPP or MSU.

Article Snippet: CPP and MSU crystals were from InVivo Gen, Toulouse, France; phorbol myristate acetate (PMA), RPMI 1640, fetal calf serum, polymyxin B and phosphate buffer saline (PBS) were from Sigma-Aldrich, Milan, Italy.

Techniques: Gene Expression, Expressing

RES and PD suppress ROS production. White columns show ROS released by THP-1 cells treated with 0.025 mg/mL CPP ( A ) or 0.05 mg/mL MSU ( B ) crystals in presence of RES and PD. Grey and black columns indicate the amount of ROS released in cultures pretreated for 2 h with PD or RES, and treated with crystals after 24 h. On the left of the figures are the control conditions; CONTROL: cells only; PD: cells with PD only; RES, cells with RES only. Values are expressed as the mean ± standard deviation.* p < 0.05 vs. MSU or CPP.* p < 0.05, ** p < 0.01 vs. CPP or MSU.

Journal: Foods

Article Title: Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells

doi: 10.3390/foods8110560

Figure Lengend Snippet: RES and PD suppress ROS production. White columns show ROS released by THP-1 cells treated with 0.025 mg/mL CPP ( A ) or 0.05 mg/mL MSU ( B ) crystals in presence of RES and PD. Grey and black columns indicate the amount of ROS released in cultures pretreated for 2 h with PD or RES, and treated with crystals after 24 h. On the left of the figures are the control conditions; CONTROL: cells only; PD: cells with PD only; RES, cells with RES only. Values are expressed as the mean ± standard deviation.* p < 0.05 vs. MSU or CPP.* p < 0.05, ** p < 0.01 vs. CPP or MSU.

Article Snippet: CPP and MSU crystals were from InVivo Gen, Toulouse, France; phorbol myristate acetate (PMA), RPMI 1640, fetal calf serum, polymyxin B and phosphate buffer saline (PBS) were from Sigma-Aldrich, Milan, Italy.

Techniques: Control, Standard Deviation

RES and PD decrease NO production. White columns show quantification of NO production by THP-1 cells treated with 0.025 mg/mL CPP ( A ) or 0.05 mg/mL MSU ( B ) crystals in presence of RES and PD. Grey and black columns indicate the amount of NO released in cultures pretreated for 2 h with PD or RES, and treated with crystals after 24 h. On the left of the figures are the control conditions; CONTROL: cells only; PD: cells with PD only; RES, cells with RES only. Values are expressed as the mean ± standard deviation.* p < 0.01 vs. MSU or CPP; ** p < 0.05 vs. MSU.

Journal: Foods

Article Title: Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells

doi: 10.3390/foods8110560

Figure Lengend Snippet: RES and PD decrease NO production. White columns show quantification of NO production by THP-1 cells treated with 0.025 mg/mL CPP ( A ) or 0.05 mg/mL MSU ( B ) crystals in presence of RES and PD. Grey and black columns indicate the amount of NO released in cultures pretreated for 2 h with PD or RES, and treated with crystals after 24 h. On the left of the figures are the control conditions; CONTROL: cells only; PD: cells with PD only; RES, cells with RES only. Values are expressed as the mean ± standard deviation.* p < 0.01 vs. MSU or CPP; ** p < 0.05 vs. MSU.

Article Snippet: CPP and MSU crystals were from InVivo Gen, Toulouse, France; phorbol myristate acetate (PMA), RPMI 1640, fetal calf serum, polymyxin B and phosphate buffer saline (PBS) were from Sigma-Aldrich, Milan, Italy.

Techniques: Control, Standard Deviation

The effect of PD and RES on CPP and MSU crystal phagocytosis. ( A ) cells stimulated with CPP crystals and treated (square) or pretreated (triangle) with PD; ( B ) cells stimulated with CPP crystals and treated (square) or pretreated (triangle) with RES; ( C ) cells stimulated with MSU crystals and treated (square) or pretreated (triangle) with PD; ( D ) cells stimulated with MSU crystals and treated (square) or pretreated (triangle) with RES. Phagocytosis was evaluated using ordinary/polarized light microscopy assessing the presence of intracellular crystals at the indicated time points. Crystals were used at the concentration of 0.025 (CPP) and 0.05 mg/mL (MSU). * p < 0.05 vs. CPP.

Journal: Foods

Article Title: Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells

doi: 10.3390/foods8110560

Figure Lengend Snippet: The effect of PD and RES on CPP and MSU crystal phagocytosis. ( A ) cells stimulated with CPP crystals and treated (square) or pretreated (triangle) with PD; ( B ) cells stimulated with CPP crystals and treated (square) or pretreated (triangle) with RES; ( C ) cells stimulated with MSU crystals and treated (square) or pretreated (triangle) with PD; ( D ) cells stimulated with MSU crystals and treated (square) or pretreated (triangle) with RES. Phagocytosis was evaluated using ordinary/polarized light microscopy assessing the presence of intracellular crystals at the indicated time points. Crystals were used at the concentration of 0.025 (CPP) and 0.05 mg/mL (MSU). * p < 0.05 vs. CPP.

Article Snippet: CPP and MSU crystals were from InVivo Gen, Toulouse, France; phorbol myristate acetate (PMA), RPMI 1640, fetal calf serum, polymyxin B and phosphate buffer saline (PBS) were from Sigma-Aldrich, Milan, Italy.

Techniques: Light Microscopy, Concentration Assay